1.
strand 1: AGTTCCGATATCGCTC
strand 2: GCAGTCGTAGCGATGTA
strand 3: ATCGCTCGATCGACTACATCGC
AGTTCCGATATCGCTC
__________ATCGCTCGATCGACTACATCGC
________________________________GCAGTCGTAGCGATGTA
2. In my oppinion I would think that the shotgun method would be more productive because there has been no previous sequencing done on this fungus and the shotgun method is a brute-force method and would probably take fewer sequencing reactions then creating a physical map first and sequencing from there. (This is just my guess though, if anyone else has an oppinion please post.)
3. The Drosophila genome is 20% exons, 16.7% introns and 63.3% intergenic sequence.
4. What probably happened was that were a known number of genes within the Drosophila genome and once the genes and their locations were identified along with the bases around them in the genome they decided there was no need to sequence 60 million exta bases of junk DNA.
Subscribe to:
Post Comments (Atom)
1 comment:
Hey- i think for Question #2 from Feb. 5th (or as you have it listed here as 4th), the quicker way IS the shotgun sequencing, but shotgun sequencing also has more sequencing reactions b/c in the physical map, you can put it in order. With the entire sudden shotgun, you don’t know the order (so it is more redundant?)
I think this is right...we went over it in lab.
Post a Comment