Questions from January 29, 2007

1.
a. the restriction enzyme EcoR1 would only cut the plasmid in one place creating a single 6 kb strand. When put through gel electrophoresis the strand would not travel very far because it is such a large strand.
b. the restriction enzyme BamH1 would cut the plasmid in two locations creating a 1.5 kb strand and a 4.5 kp strand. When put through gel electrophoresis the 1.5 kp strand would travel quickly through the gel while the 4.5 kp strand would move more slowly but farther than a 6 kp strand.
c. if both restriction enzymes are used the plasmid is cut in three places creating 2 1.5 kb strands and one 3 kb strand. When put through gel electrophoresis the 1.5 kb strands would move quickly through the gel while the 3 kb strand moves slightly slower, you would not be able to tell the two 1.5 kb pairs apart however.

2. Sal1 is compatible with Xho1
Xba1 is compatible with Spe1
Kas1 is not compatible with Bbe1 because the sticky ends of the two cuts are identical, not compliments, both are CGCG